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yeast expression vector pact2  (TaKaRa)


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    Structured Review

    TaKaRa yeast expression vector pact2
    Yeast Expression Vector Pact2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vectors+pact2/pm33798946-46-13-17
    Average 86 stars, based on 1 article reviews
    yeast expression vector pact2 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Expressing:

    Article Title: Identification and characterization of the Hesr1/Hey1 as a candidate trans-acting factor on gene expression through the 3' non-coding polymorphic region of the human dopamine transporter (DAT1) gene.
    Article Snippet: Expression of the dopamine transporter (DAT) gene affects dopaminergic neurotransmission.. A variable number tandem repeat (VNTR) polymorphism in the 3′ noncoding region of the human DAT (DAT1) gene has been reported to affect gene expression as a cis-element, and is associated with some neuropsychiatric disorders.. In this study, we identified the basic helix-loop-helix (bHLH) transcriptional factor Hesr1 (the Hairy/enhancer of split related transcriptional factor 1 with a YRPW motif, also named Hey1/HERP2/HRT1/CHF2) as a trans-acting factor in a yeast one-hybrid system, and showed that Hesr1 down-regulates reporter gene expression with the 3′ noncoding region of DAT1 gene in mammalian cell lines.

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..

    Modification:

    Article Title: Identification and characterization of the Hesr1/Hey1 as a candidate trans-acting factor on gene expression through the 3' non-coding polymorphic region of the human dopamine transporter (DAT1) gene.
    Article Snippet: Expression of the dopamine transporter (DAT) gene affects dopaminergic neurotransmission.. A variable number tandem repeat (VNTR) polymorphism in the 3′ noncoding region of the human DAT (DAT1) gene has been reported to affect gene expression as a cis-element, and is associated with some neuropsychiatric disorders.. In this study, we identified the basic helix-loop-helix (bHLH) transcriptional factor Hesr1 (the Hairy/enhancer of split related transcriptional factor 1 with a YRPW motif, also named Hey1/HERP2/HRT1/CHF2) as a trans-acting factor in a yeast one-hybrid system, and showed that Hesr1 down-regulates reporter gene expression with the 3′ noncoding region of DAT1 gene in mammalian cell lines.

    Plasmid Preparation:

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..

    Construct:

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..

    Amplification:

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..

    Polymerase Chain Reaction:

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..

    Clone Assay:

    Article Title: Characterization of the Interaction between the Wilson and Menkes Disease Proteins and the Cytoplasmic Copper Chaperone, HAH1p
    Article Snippet: .. Plasmid Constructs—The entire coding region from a human liver HAH1 cDNA was amplified by polymerase chain reaction and cloned into the expression vectors pACT2 (CLONTECH), pVP16 (CLONTECH), and pProEx-HTb (Life Technologies, Inc.) to create the plasmid constructs pACT-HAH1, pVP-HAH1, and pHTb-HAH1. ..



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